General description

rProtein A Sepharose^™ 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

rProtein A Sepharose^™ 4 Fast Flow is based on the well established Sepharose^™ Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose^™ 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.

rProtein A Sepharose^™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose^™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

High dynamic binding capacity due to oriented, single-point attachment of protein A
Well-established Protein A medium used in many approved MAb processes
No mammalian components involved during the manufacturing process