Ru360 - Calbiochem (C15-1305-718)

General description

A cell-permeable oxygen bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 0.34 nM) and specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (for complete block after incubation with ~10 µM of Ru360 for 30 min. Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction even at micromolar levels.

A cell-permeable oxygen-bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 340 pM). Specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (complete block after incubation with ~10 µM of Ru360 for 30 min). Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction, even at micromolar levels.

Note: 1 set = 10 x 100 µg.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Ca2+ uptake into mitochondria in vitro

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 184 pM blocking Ca2+ uptake into mitochondria in vitro; and in situ in intact myocytes; Kd = 340 pM for binding mitochondria

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in solution; reconstitute just prior to use.

Other Notes

Sanchez, J.A., et al. 2001. J. Physiol. 536, 387.
Bassani, R.A., et al. 1998. Cell Calcium23, 433.
Matlib, M.A., et al. 1998. J. Biol. Chem.273, 10223.
Zhou, Z., et al. 1998. J. Physiol.507 (pt 2), 379.
Emerson, J., et al. 1993. J. Am. Chem. Soc.115, 11799.
Ying, W.-L., et al. 1991. Biochemistry30, 4949.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 550.78. Empirical Formula: C₂H₂₆Cl₃N₈O₅Ru₂. Quality Level: 100. Assay: ≥. 97%. form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: green. solubility: deoxygenated water: 0.5 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.