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S1814.PB5 HUMAN EMBRYO FIBROBLAST (C005B-234364)

Catalog No.
C005B-234364
Mfr. No.
85011402-1VL
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
VI
Price: $1,835.55
List Price: $2,039.50

Cell Line Origin Human embryo fibroblast Cell Line Description Human embryo fibroblast cell line. DNA Profile STR-PCR Data: Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 11 D5S818: 13 D7S820: 10,11 THO1: 7,9 TPOX: 9,11 vWA: 15 Culture

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Cell Line Origin

Human embryo fibroblast

Cell Line Description

Human embryo fibroblast cell line.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human embryo. growth mode: Adherent. karyotype: Diploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:
12352202
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
85011402-1VL
Temperature Control Device:
Yes

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