SAG dihydrochloride (C09-1171-165)

SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist ( EC 50 =3 nM; K d =59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine ( HY-17024 ) inhibition of Smo .Appearance:SolidIC50& Target:EC50: 3 nM (Smo)In Vitro:SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC 50 of 3 nM and then inhibits expression at higher concentrations. SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing CosIn Vivo:SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice. SAG (15-20mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice.Biological Activity:SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist ( EC 50 =3 nM; K d =59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine ( HY-17024 ) inhibition of Smo. Molecular Formula: C28H30Cl3N3OS. Molecular Weight: 562.98.