SalI

SalI is a Type IIP restriction enzyme, which can recognize G^TCGAC palindromic sequence, and is suitable for enzyme cleavage plasmid, PCR products, etc., and can complete DNA cleavage reaction within 15-30 min. Recombinant albumin was added to both SalI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.SourceE.coliEnzyme Activity DefinitionIn a 50 μl reaction system, the amount of enzyme required for enzymatic digestion of 1 μg λDNA (HindIII enzyme digestion) within 1 hour at 37℃ is defined as 1 unit of activity (U).Restriction Enzyme CuttingSite5’…G↓TCGAC…3’3’…CAGCT↑G…5’Protocol(1) Configure the reaction system, as shown in the following tableComponentvolumeDNATo 1μg10×Cut Reaction Buffer5 μlSalI1 μl*Nuclease-free WaterTo 50μl(2) Mix well and incubate at 37℃ for 15-30min.(3) Incubation at 65°C for 20 min terminated the reaction.Note :*Sal is finally added to the system, the general 50u reaction system adds 1μl of Sall, and can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity due to high glycerol content.Matters needing attention(1)Sall is not sensitive to Dam and Dcm methylation; It is sensitive to CpG methylation.(2) Before the enzyme digestion reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion.(3) This product is for scientific research only and shall not be used for other purposes.

Specifictaions: BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, for Southern blotting, 20 U/μl

Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/L397686-SCI_8506fe0d91676d9d3d37aa1c0fa5c7e9.pdf