Cell Line Origin
Human primary osteogenic sarcoma
Cell Line Description
Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.
Application
SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human bone. growth mode: Adherent. karyotype: 2n : 46, (P1) Hyperploid to hypopentaploid. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42296705
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89050205-1VL