Double dropout formulation of Synthetic Defined (SD) Yeast Media which includes all necessary components for selective growth of Saccharomyces cerevisiae. Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the SD Medium-Leu-Trp formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17g per liter of agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium or plate medium by adding 27.4g of SD Medium-Leu-Trp only or 27.4g of SD Medium-Leu-Trp and 17g Agar to 1000ml of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 15 minutes. After cooling to 50°C, plates of SD Medium-Leu-Trp with Agar were poured.
- UPC:
- 42171501
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 114823065
