SD Medium-URA (Pouches), 10 x 0.5L

SD Medium-URA is a single dropout synthetic medium for selective growth of Saccharomyces cerevisiae . Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae . MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. Applications Dropout Synthetic Defined Media used to select for yeast containing plasmids with specific nutritional markers and to select for gene function in transformation experiments. Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the SD Medium-Ura formula. DOB (Drop Out Base) is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate, 20 g Dextrose per liter. DOBA is DOB with 17 g per liter of agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare plate medium by adding 27.4 g of SD Medium-Ura and 17 g Agar to 1000 mL of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 15 minutes. After cooling to 50 °C plates were poured. Recommended Use Add contents of the pouch (13.7 g) to 500 mL or (27.4 g) to 1000 mL of distilled, deionized water. Add agar if pouring plates. Autoclave at 121 °C for 15 minutes for complete synthetic defined single drop-out medium for the growth of S.cerevisiae .