Sephadex® G-50

Description General description: Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations. Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required. Features and Benefits: Quickly desalts, removes contaminants and transfers to a new buffer in a single step. Suitable for purification of DNA from small molecules by gel filtration Larger bead size for large scale group separations where high flow rates and low operating pressures are required. Classic gel filtration medium. Storage and Stability: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. Store at 4 to 30 °C () Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com. Legal Information: Sephadex is a registered trademark of Cytiva

1. What is Sephadex® G-50 used for? It is used for desalting, buffer exchange, and purification of biomolecules with molecular weights over 30,000. 2. What is the base material of Sephadex® G-50? Sephadex® G-50 is made by crosslinking dextran with epichlorohydrin. 3. What are the available particle sizes for Sephadex® G-50? It is available in Course, Medium, Fine, and Superfine grades to suit various separation needs. 4. Can Sephadex® G-50 be used for DNA purification? Yes, it is suitable for the purification of DNA from small molecules by gel filtration. 5. What are the storage conditions for Sephadex® G-50? Store at 4 to 30 °C; note that it may be shipped up to 90 days before its expiration date.