General description
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag
Biochem/physiol Actions
Proliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered PBS, BSA.
Reconstitution
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
Other Notes
Hoang, et al. 1996. J. Biol. Chem.271, 26131.Leyns, et al. 1997 Cell88, 747.Wang, et al. 1997 Cell88, 757.Mayr, et al. 1997 Mech. Dev.63, 109.Rattner, et al. 1997. PNAS94, 2859.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: ≤. 1 . EU/μ. g Endotxin. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2.- UPC:
- 51183709
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 565834-10UG
