sFRP-3, His.Tag , Mouse, Recombinant (C15-1305-825)

General description

Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.

Biochem/physiol Actions

Proliferation Assay:

ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.



Functional ELISA:

50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.

Other Notes

Hoang, et al. 1996. J. Biol. Chem.271, 26131.Leyns, et al. 1997 Cell88, 747.Wang, et al. 1997 Cell88, 757.Mayr, et al. 1997 Mech. Dev.63, 109.Rattner, et al. 1997. PNAS94, 2859.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. impurities: ≤. 1 . EU/μ. g Endotxin. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2.