Cell Line Origin
Rabbit Cornea
Cell Line Description
Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Application
Virus studies: Rubella virus
Culture Medium
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37°C.
Other Notes
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biological source: rabbit eye. growth mode: Adherent. karyotype: 2n : 44. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181888
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89090404-1VL