Cell Line Origin
Human Caucasian lung squamous carcinoma
Cell Line Description
Derived from the pleural effusion of a 65 year old Caucasian male with squamous cell carcinoma of the lung. HLA = A3, B7, B27, w30.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human lung. growth mode: Adherent. karyotype: 2n : 46. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51161515
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93120837-1VL