Cell Line Origin
Human neuroblastoma
Cell Line Description
SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.
Application
Carcinogenesis studies, cytotoxicity assays.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17
Culture Medium
DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Clumped cells have tendency to float.
Other Notes
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biological source: human nerve (bone marrow metastasis). growth mode: Adherent/aggregates in suspension. karyotype: Aneuploid. morphology: Epithelial-like. products: Not specified. receptors: Transferrin receptor. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42296606
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94092304-1VL