Skim Milk HiVegT Agar is for cultivation and enumeration of (C08-0443-481)

Skim Milk HiVeg Agar is prepared by completely replacing animal based peptones with vegetable peptones which makes the medium free of BSE/TSE risks. This medium is the modification of the medium recommended by APHA (1) for cultivation and enumeration of microorganisms encountered in dairy industry (2). Skim Milk HiVeg Agar like the conventional medium can be used for the demonstration of coagulation and proteolysis of casein (3). Skim Milk is sometimes used as a complete medium or as an ingredient in other medias for propagation of microorganisms present in contaminated milk products like Mycobacterium tuberculosis, Corynebacterium diphtheriae etc. Proteolytic bacteria hydrolyze casein to form soluble nitrogenous compounds indicated as clear zones surrounding the colonies. HiVeg hydrolysate and yeast extract provide the essential nitrogenous nutrients, carbon, sulphur, vitamin B complex and trace elements to the organisms. Dextrose serves as carbohydrate source. Skim milk serves as a good source of casein. Directions: Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.