SKL2001 (C09-1044-375)

InformationSKL2001 SKL2001 is a novel agonist of the Wnt/β-catenin pathway . It disrupts the Axin/β-catenin interaction.TargetsWnt/β-catenin (Cell-free assay)In vitroSKL2001 upregulated β-catenin responsive transcription by increasing the intracellular β-catenin protein level and inhibited the phosphorylation of β-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3β enzyme activities. SKL2001 disrupted the Axin/β-catenin interaction, which is a critical step for CK1- and GSK-3β-mediated phosphorylation of β-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/β-catenin pathway. SKL2001 did not affect either NF-κB or p53 reporter activity and inhibits β-catenin phosphorylation without affecting GSK-3β activity.Cell Research(from reference)Cell lines：HEK293 reporter and control cell lines Concentrations：20 μM Incubation Time：15 h. Specifications and Purity: 99%. Molecular Formula: C14H14N4O3. Molecular Weight: 286.29. PubChem CID: 16003447. Isomeric SMILES: C1=COC(=C1)C2=CC(=NO2)C(=O)NCCCN3C=CN=C3. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/S413970-SCI_eec69bbbbd13b59e53abc78fab525829.pdf.