General description
Interleukin-1 alpha and beta (IL-1α and IL-1β) are pro-inflammatory cytokines involved in immune defense against infection, and are part of the IL-1 superfamily of cytokines. Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. IL-1 is involved in various immune responses with a primary role in inflammation, making it a target for Rheumatoid Arthritis (RA). IL-1α and IL-1β are produced as precursor peptides, which are proteolytically processed and released in response to cell injury, and thus induce apoptosis. IL-1β production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever.
The Singulex Erenna SMC® IL-1α Immunoassay Kit contains all reagents required to perform assays on the Erenna Immunoassay System. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna Immunoassay System detects analytes using Single Molecule Counting (SMC®) technology.
Application
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of the Single Molecule Counting (SMC®) gives the SMC® Human IL-1α Immunoassay Kit unparalleled performance in the quantification of IL-1α in human EDTA plasma.
Components
IL-1α Coated Beads
Standard Diluent
IL-1α Detection Antibody
Assay Buffer
IL-1α Standard
Erenna IL-1α Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Analysis Note
Low-end CV% Range: 2 - 19%|Low-end CV% Average: 7%
Legal Information
SMC is a registered trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Shipping Information:
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- UPC:
- 41116127
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 03-0072-00
- Temperature Control Device:
- Yes
