SMOOTH MUSCLE CELL BASAL MEDIUM 2 (PRF) (C15-1346-107)

General description

Smooth Muscle Cell Basal Medium 2, phenol red-free (supplements sold separately)

Application

Smooth Muscle Cell Growth Medium 2 is a low-serum (5% V/V) medium developed to establish and maintain smooth muscle cell cultures from blood vessels and hollow organs. The medium is optimized for primary human cells but can also be used for bovine, porcine, murine, dog, and rat smooth muscle cells.Recommended for:Human Umbilical Artery Smooth Muscle Cells (HUASMC)Human Coronary Artery Smooth Muscle Cells (HCASMC)Human Pulmonary Artery Smooth Muscle Cells (HPASMC)Human Aortic Smooth Muscle Cells (HAoSMC)Human Bronchial Smooth Muscle Cells (HBSMC)Human Tracheal Smooth Muscle Cells (HTSMC)Human Uterine Smooth Muscle Cells (HUtSMC)

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Smooth Muscle Cell Growth Medium are subjected to comprehensive quality control tests using primary human smooth muscle cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested smooth muscle cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 0.5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 2 ng / mlInsulin (recombinant human): 5 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Subculture Routine

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