Smurf1, active (C15-1301-386)

General description

Smurf1 is a member of the Nedd4-like family of E3 ligases. It contains an amino terminal C2 domain and 2 WW domains that are responsible for cellular localization and substrate recognition, and a catalytic carboxyl terminal HECT domain.
Smurf1 was originally identified as a negative regulator of the TGF-β/BMP (bone morphogenetic proteins) signalling pathway by degrading SMAD proteins and TGF-β /BMP receptors. To date, over a dozen substrates have been identified, including Runx2, MEKK2, RhoA, Par6, JunB, Talin head, Prickle1, TRAF family proteins, and STAT1. This suggests a pleiotropic role for Smurf1 in various cellular functions such as bone homeostasis, embryonic development, cell movement and polarity control, and immune response. Aberrant expression of Smurfs has been shown to occur in several types of cancers, including breast, oesophageal, pancreatic and renal cell carcinomas.

Application

Ubiquitination Cascade Comoponent: E3

Physical form

Purified using glutathione sepharose.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: human. Quality Level: 100. form: liquid. manufacturer/tradename: Upstate^®. . technique(s): activity assay: suitable. NCBI accession no.: NM_181349. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.