Cell Line Origin
Human Colorectal Carcinoma
Cell Line Description
This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate
Subculture Routine
Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human intestine. growth mode: Semi-adherent aggregates. karyotype: Pseudodiploid. Modal chromosome number is 46 at 70% with polyploidy at 16.4%. The del(3) (p23), t(4,?), t(8. ?) (p22), del (9) (p13), del (11) (p13), t(1. 8) (p13. p24), t(1. ?) (q11,?) and 2 other marker chromosomes, if any, occur only sporadically.. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41115709
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96020932-1VL
