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Gill-Hematoxylin Stain (Modified) - Solution I (950mL)

ENG Scientific

Catalog No.
ENG 8411
Manufacturer No.
8411
Manufacturer Name
ENG Scientific
Quantity
1
Unit of Measure
BO
Price: $139.00
List Price: $152.90

A bottle of Modified Gill-Hematoxylin Stain (Solution I), commonly used in histology laboratories for nuclear staining of tissue sections, supporting the visualization of cell nuclei and histological analysis in research and diagnostic applications.

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  • Solution I - Gill-Hematoxylin, 0.2% (Modified), 0.4% or 0.6%
  • Solution II - Scott’s Tap Water Substitute
  • Solution III - Ethanol (95%)

An excellent nuclear stain employing a half-oxidized hematoxylin solution. (Gill, 1974) Gill Hematoxylin is a high contrast stain and a progressive hematoxylin which enchances the use of counterstains. To achieve desired stain characteristics in different specimens, the stain is available in 3 concentrations at 0.2% w/v, (Modified), 0.4% w/v, and 0.6% w/v. The modified solution at 0.2% may be preferred in cytology, however, by increasing the time (10 minutes) it can be used in histology. Gill-Hematoxylin at 0.4% is recommended for histology, however, a shorter stain time renders this solution suitable for cytologic use. For comparable results to those obtained with Harris’ Hematoxylin, Gill-Hematoxylin at 0.6% is recommended. An essential part of this stain sequence is the use of a blueing agent. We recommend Scott’s Tap Water Substitute which ensures a controlled pH and aids in cell retention on slides.

CONTENTS: Ethylene Glycol, Hematoxylin, Sodium Iodate, Aluminum Sulfate, Citric Acid, Magnesium Sulfate, Sodium Bicarbonate, Ethanol

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Gill-Hematoxylin (Modified): Fix in Sol. III for 15 minutes. Water - 2 x (10 dips each). Gill-Hematoxylin (Modified) - 2 minutes. Water - 2 x (10 dips each). Sol. II - 1 minute. Water - 2 x (10 dips each). Sol. III - 2 x (10 dips each). Continue with preferred staining method. Gill-Hematoxylin 0.4 % or 0.6 %: Xylene - 3 x (2 minutes each). Absolute Ethanol - 3 x (30 seconds each). Water - 2 x (10 dips each). Gill Hematoxylin 0.4% or 0.6% - 3 minutes. Water - 2 x (10 dips each). Sol. II - 2 minutes. Water - 2 x (10 dips each). Continue with preferred staining method.

RESULTS: Nuclear chromatin stains blue to blue-black; cytoplasm faintly tinted.

Safety Data Sheet - Gill-Hematoxylin Stain Kit, Modified (Solution I)

UPC:
41116105
Condition:
New
Availability:
3-5 Days
Weight:
2.25 Ounces
HazMatClass:
No
WeightUOM:
LB
MPN:
8411
Product Size:
950mL

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