Solution I - Carbol Fuchsin
Solution II - Acid Alcohol
Solution III - Concentrated Light Green

This technique aids in differentiating Crytosporidium oocysts from yeast cells.

CONTENTS: Basic Fuchsin, Ethanol, Phenol, Glacial Acetic Acid, HCl, Light Green SF

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700). Formalin-preserved Specimens: Mix stool suspension thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Centrifuge for 2 minutes, decant supernatant and add saline to the remaining sediment. Centrifuge again and decant saline. Smears may be prepared from upper layer of sediment. Material should be spread on a clean glass slide and allowed to air dry. Fix fresh specimens in methanol and formalized specimens with heat. Flood smear with Sol. I for 1 - 2 minutes. Rinse gently with water. Decolorize with Sol. II and rinse again with water. Counterstain with Sol. III for 30 - 60 seconds. Rinse with water and allow to dry. NOTE: Decolorization and counterstaining times may vary according to thickness of specimen.

RESULTS: Cryptosporidium oocysts - light pink to deep red; yeast cells - green to blue-purple.

Safety Data Sheet - Cryptosporidium Stain Kit (Solution II)