Solution I - Eosin Y, (1%) Aqueous
Solution IIA - Crystal Violet
Solution IIB - Aniline Oil (2.3%) Aqueous
Solution III - Grams Iodine
Solution IV - Aniline Oil/Xylene
Solution V - Xylene

This stain kit is useful in demonstrating Pneumocystis carinii in smears of sputum, transtracheal aspirates, and bronchial washings, however, lung biopsy specimens obtained by percutaneous needle aspiration provide a better opportunity for diagnosis. Tissue sections and imprints may also be used. Prior to staining, imprints and smears must be air-dried and fixed in methanol.

CONTENTS: Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Working Solution: Mix 10 ml of Sol. IIA with 90 ml of Sol. IIB. Stain air-dried smears or imprints in Sol. I for 3 - 5 minutes. Rinse with water. Stain in working solution for 5 - 10 minutes. Rinse in Sol. III for 5 - 10 minutes. Rinse in water and blot dry. Decolorize in Sol. IV and rinse in Sol. V.

RESULTS: Pneumocystis appears blue to deep purple against a pink background.

Safety Data Sheet - Gram-Weigart Stain Kit (Solution IIB)