Solution I Sudan Black B
Solution II Buffer
Solution III 70% Ethanol
Solution IV Harris' Alum Hematoxylin

This technique may be substituted for the benzidine-peroxidase method used for the differential diagnosis of acute granulocytic leukemia, acute lymphocytic and acute myelo-monocytic leukemia.

CONTENTS: Sudan Black B, Ethanol, Buffers, Stabilizers, Hematoxylin, Potassium Alum, Mercuric Oxide

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Working Solution: Prepare as needed by mixing 60 ml of Sol. I with 40 ml of Sol. II. Filter, using suction. Fixation: Place several pieces of filter paper in the bottom of a Coplin jar with screw-top lid. Moisten with 3 - 4 drops of 37% formaldehyde. Place air-dried smears in Coplin jar and cap tightly. Fix for 10 minutes. Wash in running water for 2 minutes. Place smears in working solution for 30 - 60 minutes. Wash in Sol. III for 30 - 60 seconds to remove excess stain. Wash in tap water for 2 minutes. Counterstain in Sol. IV for 10 minutes. Wash in tap water for 5 minutes.

RESULTS: Lymphocytes and normoblasts are not stained with Sudan Black B. Normal granulocyte precursors from blast cells onward show increasing sudanophilia corresponding roughly to the granules present. Promyelocytes contain a few sudanophilic granules, while mature polymorphonuclear neutrophils contain large numbers of sudanophilic granules. Auer bodies are intensely sudanophilic.

Safety Data Sheet - Sudan Black B Stain Kit (Solution III)