Solution I - Crystal Violet
Solution II - Grams Iodine
Solution III - Ethanol (70%)
Solution IIIA - Ethanol (95%)
Solution IIIB - Acetone-Alcohol
Solution IV - Safranin
Solution IVA - Basic Fuchsin
Solution IVB - Carbol Fuchsin

AVAILABLE IN VARIOUS COUNTERSTAINS AS FOLLOWS:

Safranin - Routinely used for gram negative bacteria.
Basic Fuchsin - More intense overall staining to clearly identify weak gram-negative bacteria and also aids in the detection of Bordatella.
Carbol Fuchsin - A counterstain of greater overall intensity to easily identify Legionella.

Also available with various decolorizers to suit individual preference of technique. Convenient flip-top cap for 250 ml and 950 ml sizes.

CONTENTS: Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Cover fixed smear with Sol. I for 1 minute and rinse gently with distilled water. Apply Sol. II for 1 minute and rinse gently with distilled water. Decolorize with Sol. III, Sol. IIIA, or Sol. IIIB until the purple color no longer runs off the slide, (from 2 - 20 seconds depending upon decolorizer and thickness of smear) and rinse with distilled water. Counterstain with Sol. IV, Sol. IVA or Sol. IVB for 1 minute and rinse with distilled water. Allow to dry and examine under oil immersion.

NOTE: For a faster decolorizer, use Sol.IIIB - Acetone-Alcohol. For a more intense counterstain, we recommend Sol. IVA - Basic Fuchsin or Sol. IVB - Carbol Fuchsin.

RESULTS: Gram-positive bacteria stain purple to blue black. Gram-negative bacteria stain pink to red.

Safety Data Sheet - Gram Stain Kit (Solution IIIA)