Solution I - Formalin (10%)
Solution II - Methanol Fixative
Solution III - Modified Trichrome Stain
Solution IV - Acid Alcohol
Solution V - Ethanol (95%)
Solution VI - Ethanol
Solution VII - Xylene

This stain method was designed as a simple, non-invasive technique for the rapid detection of microsporidia spores in fecal material by light microscopy. The chromotrope based stain is a modification of the trichrome stain for intestinal protozoa.

CONTENTS: Chromotrope 2R, Fast Green FCF, Phosphotungstic Acid, Glacial Acetic Acid

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Specimens for light microscopial examination should be prepared from a suspension of unconcentrated liquid stool (10 ul) in Sol. I (1:3 ratio). Smears are prepared directly and thinly spread on a glass slide covering a 45 x 25 mm area. Fix smears in Sol. II for 5 minutes. Stain smears with Sol. III for 90 minutes. Rinse slides in Sol. IV for 10 seconds. Rinse briefly in Sol. V to remove acid. Dehydrate in Sol. V for 5 minutes, Sol. VI for 10 minutes and Sol. VII for 10 minutes. Read microscopically under oil immersion lens (magnification x 1000).

RESULTS: Enterocytozoon bienuesi spores appear ovoid and refractile at approximately 1.5 by 0.90 um. The spore wall of properly stained smears should appear a bright pinkish red. Most bacteria and background debris stain a faint green.

Safety Data Sheet - Modified Trichrome Stain Kit (Solution IV)