Solution I 95% Ethanol
Solution II Sudan IV
Solution III Acetic Acid, 36%
Solution IV Loeffler's Methylene Blue

Specifically for use in the microscopic examination of fecal material for fatty acids and neutral fats, as well as fecal leukocytes.

CONTENTS: Ethanol, Acetic Acid, Sudan IV, Methylene Blue

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Neutral Fats: Place a small aliquot of fecal suspension on a clean glass slide. Mix with 2 drops of Sol. I. Add and mix 2 drops of Sol. II. Coverslip and read microscopically. Fatty Acids: Place a small aliquot of fecal suspension on a clean, glass slide. Mix with 2 drops of Sol. III. Add and mix 2 drops of Sol. II. Coverslip and heat to boiling for a few seconds. Examine microscopically under high power while still warm.

Fecal Leukocytes: Place a small aliquot of mucus or liquid stool on a clean, glass slide. Add 2 drops of Sol. IV and mix. Coverslip and allow to stand for 2 - 3 minutes before examining microscopically. NOTE: Fresh material should be used for optimum results.

RESULTS: Neutral fats form yellow to pale orange refractile globules. Fatty acids appear as deep orange globules. To determine the presence of fecal leukocytes, examine stained specimen under low power. Differential counts (mononuclear or polymorphonuclear cells only) should be performed under high power.

Safety Data Sheet - Fecal Stain Kit (Solution IV)