Solution I - PVA Solution (5%)
Solution IA - Schaudinn Solution
Solution IB - Glacial Acetic Acid
Solution II - Iodine Alcohol
Solution III - Ethanol (70%)
Solution IV - Trichrome Stain
Solution V - Acid Alcohol (90%)
Solution VI - Ethanol (95%)
Solution VII - Carbol-Xylene
Solution VIII - Xylene

NOTE: THIS STAIN IS NOT TO BE CONFUSED WITH GOMORI’S TRICHROME STAIN USED FOR HISTOLOGY. IT IS A RECOMMENDED METHOD BY THE ASCP. "MANUAL-LABORATORY DIAGNOSIS OF INTESTINAL PARASITIC INFECTION".

A simple and quick technique for staining intestinal protozoa based on the principle of the attraction that these parasites show for the Chromotrope 2R dyes.

PVA Solution, when mixed with liquid stool specimens, (3 - 4 drops PVA with 1 - 2 drops of fecal material on a slide) provides excellent adhesion of specimen to slides, if allowed to dry several hours at 35oC or overnight at room temperature. Schaudinn fixative will properly fix and hold fresh stool specimens that have been thinly spread on a slide. (5 minutes at 50oC or 1 hour at room temperature) Smears may be left in fixative for several hours or 1 - 2 days without harm. PVA fixative (a combination of PVA Solution and Schaudinn’s fixative) will adhere as well as preserve organisms and prevent their loss during staining. One distinct advantage of PVA fixative is that it makes possible the successful staining of organisms in liquid specimens.

CONTENTS: PVA Powder, Mercuric Chloride, Iodine, Ethanol, Chromotrope 2R, Light Green SF, Fast Green FCF, Phosphotungstic Acid, Acetic Acid (Glacial), Xylene, Pheno2

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIANOSTIC USE ONLY.

RECOMMENDED PROCEDURE: STAINING TECHNIQUE WITH PVA FILMS: PVA-fixed smears should be allowed to dry overnight at 37o degrees C prior to staining. Sol. II - 10 - 20 minutes. Sol. III - 3 - 5 minutes, then repeat. Sol. IV - 8 minutes. Sol. V - 10 - 20 seconds. Rinse with Sol. VI to remove acid. Sol. VI - 5 minutes. Sol. VII - 5 - 10 minutes. Sol. VIII - 10 minutes. Mount with coverslip.

STAINING TECHNIQUE WITH FRESH SPECIMENS: Schaudinn’s fixative - 5 minutes at 50oC or 1 hour at room temperature. Sol. II - 1 minute. Sol. III - 1 minute, then repeat. Sol. IV - 8 minutes. Sol. V - 3 - 10 seconds. Sol. VI - rinse briefly, 2 changes. Sol. VII - 1 minute.Sol. VIII - l.- 3 minutes. Mount with coverslip.

NOTE: CHANGE ALCOHOLS OFTEN.

RESULTS: The cytoplasm of thoroughly-fixed and well-stained cysts and trophozoites are blue-green tinged with purple. The nuclear chromatin, chromatoid bodies, ingested red cells, and bacteria stain red or purplish red. Other ingested particles, such as yeast or molds generally stain green, but variations frequently occur in the color reaction of ingested particles.Other ingested particles, such as yeasts or molds, generally stain green, but variations in color frequently occur. Background material usually stains green, and a color contrast with the protozoa results.

Safety Data Sheet - Trichrome Stain Kit (Solution IV)