Solution I - Phloxine (1%)
Solution II - Phosphotungstic Acid (3%)
Solution III - Azure II (0.05%)
Solution IVA - Weigert’s Hematoxylin
Solution IVB - Weigert’s Iron Chloride

CONTENTS: Phloxine, Phosphotungstic Acid, Azure II, Hematoxylin, Ferric Chloride, Ethanol, HCl

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Prepare working Solution IV: Mix equal parts of Sol. IVA with Sol. IV B. Sol. I -10 minutes. Rinse in distilled water. Sol. II - 1 minute. Rinse again. Sol. III - 30 seconds. Rinse again. Sol. IV - 1 minute. Rinse in distilled water. Dehydrate and clear in xylene, 2 changes each.

RESULTS: A cells - purple; B cells - violet blue; D cells - light blue with evident granules; exocrine cells - grayish blue with red granules.

Safety Data Sheet - Maldonado's Method for Pancreatic Islet Cells (Solution IVA)