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SP2/0-AG14 (SERUM-FREE)

Sigma Aldrich

Catalog No.
C15-1617-211
Manufacturer No.
08060101-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VL
Price: $1,835.55
List Price: $2,039.50

Cell Line Origin Mouse x mouse hybridoma non-secreting, serum-free, animal component (AC) free Cell Line Description Sp2/0-Ag14 cell line (Sigma Catalogue number. 85072401) adapted to grow in animal component free medium (EX-CELL Sp2/0

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Cell Line Origin

Mouse x mouse hybridoma non-secreting, serum-free, animal component (AC) free

Cell Line Description

Sp2/0-Ag14 cell line (Sigma Catalogue number. 85072401) adapted to grow in animal component free medium (EX-CELL Sp2/0 Serum-Free Medium for Sp2/0 Cells Chemically Defined, Sigma cat no 14660C). Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. Can be used as a fusion partner for generating hybridomas.

Application

Fusion partner

Culture Medium

EX-CELL Sp2/0 Serum-Free Medium (Sigma cat no. 14660C) + 8mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO)

Subculture Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C..

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

UPC:
41181531
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
08060101-1VL
Product Size:
1VL

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