Application

Spautin-1 has been used as an autophagy inhibitor:

to study its effects on vascular, glial, and neuronal alterations in the oxygen-induced retinopathy mouse model
to evaluate its pre-treatment effect on the response of canine osteosarcoma cells to doxorubicin
to study its effects on the production of interleukin (IL)-6 by oxidatively stressed dendritic cells (OS-DCs) in Luminex assay

Biochem/physiol Actions

Spautin-1 inhibits the activity of two ubiquitin-specific peptidases, USP10 and USP13, causing an increase in proteasomal degradation of class III PI3 kinase complexes, which have been shown to regulate autophagy.

Specific and potent autophagy inhibitor-1 (Spautin-1) is a small molecule, which can inhibit autophagy and induce cancer cell death. It inhibits autophagy by degrading beclin-1. Spautin-1 augments the efficiency of radiation therapy and chemotherapy in various human cancer cell lines.