General description
Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His tag, MW = 69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess the expression of sphingosine kinase 1 in oestrogen receptor-negative breast cancer. Sphingosine kinase 2 has also been used in a study to investigate the antitumor effects of sorafenib (≤ 10 μM) on four human neuroblastoma cell lines.
Unit Definition
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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- UPC:
- 51112016
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- SRP0285-20UG
- Temperature Control Device:
- Yes
