General description
A cell-permeable and selective inhibitor of NAD+-dependent deacetylase activity of Sir2 protein (IC50 = 60 µM). Creates a phenocopy of the sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Reported to act by either altering or blocking access to the acetylated histone-binding pocket.
A cell-permeable lactone derived from β-naphthol that acts as a selective inhibitor of NAD+-dependent histone deacetylase activity of Sir2 protein (IC50 = 60 µM). It creates a conditional phenocopy of a sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Acts by either altering or blocking access to the acetylated histone binding pocket.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
NAD+-dependent histone deacetylase activity of Sir2 protein
Product does not compete with ATP.
Reversible: no
Target IC50: 60 µM against NAD+-dependent histone deacetylase activity of Sir2 protein
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Bedalov, A., et al. 2001. Proc. Natl. Acad. Sci. USA98, 15113.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51281623
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 567750-5MG
