Stem Cell Factor, Human, Recombinant from Expressed in E.col (C08-0506-046)

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Human SCF is an 18.4 kDa protein containing 164 amino acid residues. Stem Cell Factor is a peptide growth factor/cytokine with broad activities, especially related to hematopoiesis. Among the many activities of SCF are the ability to act on early hematopoietic progenitor/stem cells and to stimulate the proliferation and survival of mast cells. Also, SCF is one of the most potent stimulators of multilineage progenitors (CFUGEMM) in both human and murine bone marrow cells. SCF acts synergistically with other growth factors, including erythropoietin, G-CSF, M-CSF, GM-CSF, IL-3, and IL-6, to increase the number and size of colonies of hematopoietic progenitors. SCF appears to play an important role in the survival, proliferation, or migration of primordial germ cells and melanoblasts during both development and maturation. Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.