Stepharine

Stepharine, an natural alkaloid, directly interactes with TLR4 and binds to the TLR4/MD2 complex (TLR4 inhibitor). Stepharine possesses anti-aging , anti-viral and anti-hypertensive effects.In VitroStepharine (10, 30 μM) substantially inhibits nitric oxide (NO) release as well as the mRNA and protein expression of pro-inflammatory mediators [inducible nitric oxide synthase, interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β] in LPS-activated BV-2 cells. Stepharine (10, 30 μM) inhibits LPS-induced increase of TLR4 expression, IκBα phosphorylation, and NF-κB p65 nuclear translocation. Stepharine exhibits neuroprotective effects on SH-SY5Y cells cultured with LPS-treated conditioned medium. Stepharine has also shown to inhibit cholinesterase in vitro. MCE has not independently confirmed the accuracy of these methods. They are for reference only.In VivoStepharine (500 μg/kg) inhibited NeuN + cells loss and Iba-1 + cells increase in the MCAO ischemic cortex . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Middle cerebral artery occlusion (MCAO) rat model . Dosage: 500 μg/kg (20 μL). Administration: Intranasally injected into rats 1h after MCAO. Result: Attenuated the neuronal loss induced by MCAO (MCAO+ stepharine) group.Form:Solid. Specifications and Purity: ≥98.0%. Molecular Formula: C18H19NO3. Molecular Weight: 297.35. PubChem CID: 98455. Isomeric SMILES: COC1=C(C2=C3C(CC24C=CC(=O)C=C4)NCCC3=C1)OC.