General description
Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.
Application
Enzyme Immunoassay (see comments)
Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.
Reconstitution
Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.
Other Notes
Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.
The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 106. Excitation Max: 500 nm emission max: 575 nm.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51281908
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 189737-1ML
