General description

This sulfonated azadibenzocyclooctyne-biotin derivative is a highly water soluble and versatile biotinylation reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be purified using streptavidin or avidin affinity reagents.

Application

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Features and Benefits

More efficient labeling will occur at higher temperatures and with greater excess of biotin reagent. Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. Stock solutions in DMSO have been shown to be stable in the freezer for up to 3 months.

Molecular Weight: 754.96. Empirical Formula: C₃₇H₅₀N₆O₇S₂. Quality Level: 100. form: solid. reaction suitability: reaction type: click chemistry, reagent type: cross-linking reagent. storage temp.: −. 20Â°C. SMILES string: CC[NH+](CC)CC.[O-]S(: O)(: O)C(CNC(: O)CCCC[C@@H]1SC[C@@H]2NC(: O)N[C@H]12)C(: O)NCCC(: O)N3Cc4ccccc4C#Cc5ccccc35. InChI: 1S/C31H35N5O7S2.C6H15N/c37-27(12-6-5-11-25-29-23(19-44-25)34-31(40)35-29)33-17-26(45(41,42)43)30(39)32-16-15-28(38)36-18-22-9-2-1-7-20(22)13-14-21-8-3-4-10-24(21)36. 1-4-7(5-2)6-3/h1-4,7-10,23,25-26,29H,5-6,11-12,15-19H2,(H,32,39)(H,33,37)(H2,34,35,40)(H,41,42,43). 4-6H2,1-3H3/t23-,25-,26?,29-. /m0./s1. InChI key: RACLEVCKGUBCTH-GESOYTMLSA-N. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319 - H335. Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.