Super Green II RNA gel stain

Super Green has the same effect as Sybr Green. This product is dissolved in DMSO. Because the melting point of DMSO is 18.5℃, please put it to room temperature before use to dissolve it.Features of Super Green IINon-toxic: It is a kind of flower Qing dye, which is easily biodegradable and has no carcinogenic toxicityHigh sensitivity: at least 20pg ssDNA or RNA can be detected, 25-100 times higher than EB staining methodHigh signal-to-noise ratio: strong sample fluorescence signal, low background signalSimple operation: Like EB, the dye does not degrade during the precast gel and electrophoresis; the dyeing process after electrophoresis only takes 30 minutes without decolorization or washing, and can be directly observed with a UV gel transilluminator or a visible light transilluminatorWide application range: can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel electrophoresis, polyacrylamide gel electrophoresis, pulsed electric field gel electrophoresis and capillary electrophoresis, etc.; available Stain in ssDNA or RNA.Easy to use: It has no inhibitory effect on enzymes commonly used in molecular biology (such as Taq enzyme, reverse transcriptase, endonuclease, T4 ligase, etc.)Economy: The price is cheaper than silver dyeingIntroduction to the use of Super Green II1. Paste dyeing method (the usage is the same as EB) (recommended method)(1) Add Super Green II nucleic acid dye when making gel. Cool the glue to about 50°C, and add 3~5μl Super Green II 10,000× stock solution to every 100ml of glue, and analogy with this ratio(2) Perform electrophoresis in accordance with conventional methodsNote: This method of dyeing can accurately determine the molecular weight of nucleic acid fragments, and the amount of dye is relatively small. 1ml of dye can make about 300 pieces of 100ml glueDue to the poor thermal stability of Super Green II, it cannot be added directly to the hot glue solution. It needs to wait for the solution to cool to about 50°C before adding it.Shake, shake or flip to ensure that the dye is fully mixed2. Bubble dyeing(1) Perform electrophoresis in accordance with conventional methods(2) Dilute the Super Green II 10,000× stock solution with a pH 7.0～8.5 buffer solution (such as TAE, TBE or TE) at a ratio of 10000:1, and mix it to make a 1× staining solution(3) Put the gel carefully into a suitable container, such as a polypropylene container. Slowly add enough 1× staining solution to immerse the gel. Cover the container with aluminum foil to protect the dye from light. Shake and stain at room temperature for 10-30 minutes. The staining time depends on the gel concentration and thickness. Stain the polyacrylamide gel directly on the glass plate. Pour the prepared 1× staining solution gently on the gel plate, let it evenly cover the entire gel plate, and stain for 30 minutes. The glass plates must be pre-treated with a silanization solution (to avoid dyes adsorbing on the glass surface).Note: The molecular weight of nucleic acid fragments can be accurately determined when staining with the bubble staining method. But the amount of dye is higherSuper Green II parametersEx(nm)#497Em(nm)#520. Specifications and Purity: 10,000× in .