Super Safe nucleic acid dyes (C09-1038-148)

Product descriptionSuper Safe is a new non-toxic nucleic acid dye. This unique oily molecule is a cyanine dye, and it has no mutagenicity at the gel staining concentration. It is safe to use and sensitive in detection. It can be used as a stain for various nucleic acid electrophoresis and is suitable for staining with various fragment sizes. It is perfectly compatible with standard gel imaging systems and gel observation devices excited by visible light, and is suitable for ultraviolet gel imaging systems or gel observation devices excited by blue visible light.Features:1. Safe and non-toxic: Although it can penetrate the cell membrane and enter the cell, it is a cyanine dye and has no mutagenicity at the gel staining concentration.2. High sensitivity: suitable for electrophoretic staining of fragments of various sizes, without any influence on nucleic acid migration.3. High stability: suitable for the preparation of agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature and has strong light resistance.4. High signal-to-noise ratio: the sample has strong fluorescence signal and low background signal.5. Simple operation: it does not degrade during the precast gel and electrophoresis process, and can be directly observed with a visible light gel transmission instrument.6. Wide range of applications: you can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining.7. Perfect compatibility: Suitable for UV gel imaging system excited by 254nm or gel observation device excited by blue visible light. Similar to the spectrum of SYBR Green.Storage conditions: 2-8℃, dark and dry, can be stored for 12 months.Product parameterEx(nm) :490        Em(nm) :520StepsⅠ. Agarose gel electrophoresis staining (recommended method)Add Super Safe Nucleic Acid Dye to the gel1. Gel preparation: Prepare agarose gel according to the usual operation, add concentrated 10,000x Super Safe Nucleic Acid Dye to make the final concentration in the gel 1x (for example: prepare 50ml gel, add 5μl dye), light Shake well and pour the glue.2. Perform electrophoresis according to the conventional method and observe the results (the dye will not affect the DNA migration!).Ⅱ. Bubble dyeing method1. Perform electrophoresis in accordance with conventional methods.2. Make a 3x staining solution by diluting 10,000x Super Safe Nucleic Acid Dye stock solution approximately 3,300 times into pure water with electrophoresis solution.3. Place the gel carefully in a suitable container, such as a polypropylene container. Slowly add enough 3x staining solution to immerse the gel. Shake and stain at room temperature for about 30 minutes.4. In the gel imager, the observation resultNote1. Because Super Safe has good thermal stability, it can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, shake or invert to ensure that the dye is well mixed. You can also choose to add Super Safe stock solution to agarose powder and running buffer, and then heat it in a microwave oven or other common methods to prepare agarose gel. Super Safe is compatible with all commonly used electrophoresis buffer solutions.2. If the bands are always dispersed or unsatisfactory, please use bubble dyeing method to confirm whether the problem is related to the dye. If the problem persists after staining, it means that the problem has nothing to do with the dye. Please try: reduce the agarose concentration; use a longer gel; extend the gel time to ensure a clear edge; improve the sample loading technique or choose the dyeing method.3. Super Safe has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing. For polyacrylamide gel, please use the foam dyeing method. Specifications and Purity: 10000X in DMSO. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/S266213-SCI_da4809a040aca3cf1de81274079bbf86.pdf.