Superose® 6 Increase 10/300 GL

Description General description Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. Features and Benefits Increased resolution compared with Superose 6, for higher purity and improved analysis results. Reduced runtime compared with Superose 6, for rapid results. Versatile use for both preparative and analytical purposes, especially for large proteins and complexes. Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over. Suitability Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions. Storage and Stability Store at 4 to 30 °C (20% Ethanol) Analysis Note To view the Certificate of Analysis for this product, please visit www.cytiva.com. Recommended products Discover LiChropur reagents ideal for HPLC or LC-MS analysis Legal Information Superdex is a registered trademark of Cytiva Superose is a registered trademark of Cytiva Safety Information Storage Class Code 3 - Flammable liquids

Q1: What is Superose® 6 Increase 10/300 GL used for? A: It’s a prepacked column for high-resolution size exclusion chromatography (SEC) of proteins and other biomolecules with molecular weights from 5,000 to 5,000,000 Da. Q2: What sample volumes is this column suitable for? A: It is designed for small-scale applications, handling sample volumes from 25 μl to 500 μl. Q3: What makes it better than Superose® 6? A: It offers increased resolution and shorter runtimes, which improves purity, analytical clarity, and throughput. Q4: Can this column tolerate harsh cleaning procedures? A: Yes. It is stable in a wide range of harsh buffers and cleaning agents including 0.5 M NaOH, 1% SDS, 8 M urea, and organic solvents. Q5: What is the recommended storage condition? A: Store between 4 °C and 30 °C in 20% ethanol. Q6: Is it suitable for analytical and preparative purposes? A: Absolutely. It’s ideal for both applications, especially for polishing proteins and studying large complexes or interactions.