Cell Line Origin
Human breast epithelium, SV40 transformed
Cell Line Description
SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.
DNA Profile
Not specified
Culture Medium
DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
biological source: human breast. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice.- UPC:
- 42201820
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94122105-1VL