Cell Line Origin
Human colon adenocarcinoma
Cell Line Description
Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.
Application
Tumourigenicity studies
Culture Medium
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Centrifugation and resuspension are recommended to disperse clumps.
Other Notes
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biological source: human colon. growth mode: Adherent. karyotype: 2n : 46. morphology: Epithelial. products: Carcinoembryonic antigen (CEA). receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51172009
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90102543-1VL
