Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.
Application
Tumourigenicity studies
Culture Medium
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Cells do not reach confluency but will grow in small islands.
Other Notes
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biological source: human colon. growth mode: Adherent. karyotype: 2n : 46, triploid. morphology: Epithelial. products: Carcinoembryonic antigen (CEA). receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51172009
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87071008-1VL
