T4 β-GLUCOSYLTRANSFERASE

Sigma Aldrich

Catalog No.: C15-1718-445
Manufacturer No.: SRP0416-100UG
Manufacturer Name: Sigma-Aldrich
Quantity: 1
Unit of Measure: EA

Price: $1,038.46

List Price: $1,153.85

General description T4-phage β:−:glucosyltransferase, also known as UDP glucose-DNA β:-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.

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Description
Specifications

General description

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

Application

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Biochem/physiol Actions

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg²⁺, Mn²⁺ and Ca²⁺ activate this enzyme.

Physical form

Formulated in 200 mM imidazole and 20% glycerol.

UPC:: 12352204
Condition:: New
Availability:: 3-5 Days
Weight:: 1.00 Ounces
HazmatClass:: No
WeightUOM:: LB
MPN:: SRP0416-100UG
Product Size:: 100/µG

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