Cell Line Origin
Human breast tumour
Cell Line Description
Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.
Application
T47D cell line human has been used:
- to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
- has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
- to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human breast. description: Human breast tumor. growth mode: Adherent. karyotype: 2n : 46, hypertriploid, modal no. 65. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42294303
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85102201-1VL
