T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA (1). The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I Product Includes: T5 Exonuclease 10x T5 Exonuclease Reaction Buffer
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