T7 High Yield RNA Transcription kit

Product DescriptionAs a biological macromolecule, mRNA can only be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is currently one of the most efficient transcriptional promoters, so T7 RNA Polymerase (T7 RNA Polymerase) is used. In vitro transcription can obtain more synthetic products. This kit has been optimized for a series of transcription reaction systems. The T7 RNA Polymerase is used to synthesize RNA complementary to one strand of DNA from the downstream of the template DNA T7 promoter, and a large number of RNA molecules can be obtained simply and quickly.One reaction in this kit can produce 150-200μg RNA. The synthesized RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.Product components Product composition Volume Enzyme Mix 50μl 10×Transcription Buffer 200μl ATP (100 mM) 80μl UTP (100 mM) 80μl GTP (100 mMI) 80μl CTP (100 mM) 8opμl Control template ( 100ng/μl) 10μl Lithium Chloride Precipitation Solution 0.75ml×2 DNase I, RNase-free(1U/μl) 50μl RNase Free Water 1ml Product Usage1) Synthesize single-stranded RNA2) Synthesize highly specific RNA probes3) Synthesis of siRNA precursors4) Make the precursor of RNA splicing reaction (RNA splicing).