Taq DNA Polymerase (Recombinant), 1000 Units

Source: E.Coli Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ~3 direction in the presence of magnesium and also possesses a 5 ~3 exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions. Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200 M each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. 10X Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP. 10X Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution. Features: Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction Conditions: Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested Enzyme: Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account. Applications: PCR, 3 A-tailing of blunt ends, compatible with Vectors. Storage Conditions: Stable for 5 days at 10°C, for longer period of time store at -20°C. Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100. References: Chien, A. et al. (1976) J. Bacteriol. 127, 1550-7. Kaledin, A.S. et al. (1980) Biokhimiia 45, 644-51. Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.