TAQ PLUS DNA POLYMERASE, 1000U

Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. PCR products, amplified up to 20kb in length with Taq Plus DNA Polymerase, contain a mixture of blunt ends and single base (A) 3’ overhang. The error rate of this PCR amplification is 7.5x10 -5 per nucleotide per cycle. STORAGE BUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl₂, 1mM DTT, 0.1% Nonidet® P-40,0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X PCR BUFFER 100mM Tris-HCl (pH8.8), 500mM potassium chloride, 1% Triton® X-100, 16mM MgCl₂. UNIT DEFINITION One unit (U) is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate. Features 5’→3’ polymerase activity 3’→5’ proofreading exonuclease activity Error rate7.5x10 -5 nucleotide -1 cycle -1 Available as a 2X Mastermix