Tartoff - Hobbs Broth (Terrific Broth) is for the cultivation of recombinant strains of Escherichia coli. Pack size is 5KG

Tartoff Hobbs developed the Terrific Broth Medium for cultivation of recombinant Escherichia coli strains. These strains have extended growth phase when cultivated in this medium (1). Tartoff-Hobbs Medium supports high cellular density and mass and maintains the growth in the logarithmic phase for a long time. Due to this fact, it provides greater yields of recombinant proteins and plasmid DNA. Often, Tartoff-Hobbs (Terrific) Broth substitutes Luria Bertani Broth (M1151), to get enhanced yields of plasmid DNA and recombinant proteins. The procedures for inoculation, incubation and generation of recombinant strains are detailed by Sambrook et al (2). Casein enzymic hydrolysate and yeast extract supply the necessary nutrients and cofactors for the excellent growth of recombinant strains of E. coli . The addition of extra peptone and yeast extract in the medium allows higher plasmid yield per volume. The two phosphates provide good buffering action to the medium. Glycerol is used as the carbohydrate source. Unlike glucose, glycerol is not fermented to acetic acid. Directions: Suspend 47.6 grams in 1000 ml distilled water containing 4 ml glycerol. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.