Tartoff - Hobbs HiVeg Broth (Terrific HiVeg Broth) is for the cultivation of recombinant strains of Escherichia coli. Pack size is 5KG

This medium is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptone. Terrific HiVeg Broth is the modification of Terrific Broth which is a very nutritious medium designed by Tartoff and Hobbs (1) to improve the yield of plasmid in Escherichia coli. Sambrook et al (2) further recommended the protocol for the inoculation and incubation of organisms. HiVeg hydrolysate and yeast extract supply the necessary nutrients and cofactors for the excellent growth of recombinant strains of Escherichia coli. Recombinant strains have an extended growth phase in this medium. High concentration of yeast extract and HiVeg hydrolysate in the medium facilitates higher cell yields. Phosphates provide good buffering action to the medium and prevent cell death due to a drop in pH. Glycerol serves as the carbon and energy source to the organisms. Unlike glucose, glycerol is not fermented to acetic acid. Directions: Suspend 47.6 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Add 4 ml of glycerol to the medium. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.